Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain

The purpose of this study was to explore the possibility replacing an expensive yeast extract contained in fermentation medium for D-lactic acid (D-LA, R-lactic acid) biosynthesis with alternative nitrogen source. screening studies were conducted under stationary conditions and showed that pea seed hydrolysate most beneficial substrate process D-LA by strain Sporolactobacillus laevolacticus DSM 442 among used inorganic organic sources, waste materials, food agricultural products. After 96 h, 75.5 g/L obtained batch cultures a containing hydrolysate, average productivity 0.79 g/L/h, yield 75.5%, optical purity 99.4%. In fed once, analogous composition, 122.6 after 120 yield, 87.6%, 1.021 99.6%, respectively. Moreover, amount enriched above-mentioned cheap product similar amounts both bioreactor cultures. Our research shows hydrolyzed seeds, which belong legume family, may be promising source production on industrial scale.